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In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)


Citation

Niknejad, Azadeh and Abdul Kadir, Mihdzar and Kadzimin, Saleh (2011) In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae). African Journal of Biotechnology, 10 (56). art. no. BBDEBF635675. pp. 11808-11816. ISSN 1684–5315

Abstract

Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world. Phalaenopsis is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for the production of cut flowers and potted plants. Most of them have different and interesting morphological characteristics which have different value to the breeders. Phalaenopsis gigantea is one of the most difficult to grow and has the potential of producing beautiful hybrids. An efficient and reproducible method for large-scale propagation of Ph. giganteausing leaf sections has been developed. Leaf sections from in vitro young plants were cultured on New Dogashima Medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mg/L) alone and in combinations with (auxins a-naphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mg/L). The explants developed calli and protocorm-like-bodies (PLBs) within 6 weeks of culture. Treatment TDZ in combination with auxins was found to be the best for the induction of callus and PLBs. In vitroregeneration of Ph. gigantea PLB was achieved by exposure to light and transferring to hormone free NDM solid medium.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Publisher: Academic Journals
Keywords: Phalaenopsis; PLBs; New dogashima medium; Regeneration
Depositing User: Nur Farahin Ramli
Date Deposited: 30 Jan 2014 14:47
Last Modified: 14 Nov 2017 08:34
URI: http://psasir.upm.edu.my/id/eprint/23770
Statistic Details: View Download Statistic

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