Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos.

Citation

Jafari, Shahram and Hosseini, Sayyed Morteza and Hajian, Mehdi and Forouzanfar, Mohsen and Jafarpour, Farnoosh and Abedi, Parvaneh and Ostadhosseini, Somayyeh and Abbasi, Hassan and Gourabi, Hamid and Shahverdi, Abdolhossein H. and Vosough, Ahmad Dizaj and Anjomshoaa, Maryam and Haron, Abd Wahid and Nordin, Norshariza and Yaakub, Halimatun and Nasr Esfahani, Mohammad Hosein (2011) Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. Journal of Assisted Reproduction and Genetics, 28 (11). 1119 - 1127. ISSN 1058-0468; ESSN: 1573-7330

Abstract

Purpose To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos. Methods Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos. Results Irrespective of TSA treatment, POU5F1 expression was not detected until 8–16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly increased POU5F1 expression, and the yield and quality of cloned embryo development compared to control. Conclusion The POU5F1 expression of cloned embryos is strictly controlled by the stage of embryo development and may not be altered by TSA-mediated changes occur in DNA-methylation and histone-acetylation of the genome.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Agriculture Faculty of Medicine and Health Science Faculty of Veterinary Medicine
DOI Number:	https://doi.org/10.1007/s10815-011-9638-1
Publisher:	Springer
Keywords:	Epigenetic modification; POU5F1; Bovine; Cloned embryos.
Depositing User:	Nur Farahin Ramli
Date Deposited:	30 Jan 2014 07:44
Last Modified:	06 Oct 2015 07:56
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10815-011-9638-1
URI:	http://psasir.upm.edu.my/id/eprint/23763
Statistic Details:	View Download Statistic

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