Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia.

Citation

Nazerian, Eisa and Sijam, Kamaruzaman and Mior Ahmad, Zainal Abidin and Keshavarz, Kavous (2011) Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. Journal of General Plant Pathology, 77 (5). pp. 292-294. ISSN 1345-2630; ESSN: 1610-739X

Abstract

Dark brown, necrotic pods with extensive water-soaked lesions caused by plant pathogenic bacteria were found on okra plants in different fields in Malaysia in 2010. PCR amplification of the pectate lyase (pel) gene and amplification of the 16S–23S rRNA (ITS) with G1 and L1 primers produced 434-, 535- and 570-bp fragments, respectively. From the similarity between the results of biochemical tests and their equivalency with standard bacteriological sources, PCR-based pel gene, and RFLP analysis of the ITS-PCR products, all isolates were identified as Pectobacterium carotovorum. This is the first report of P. carotovorum in okra from Malaysia.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Agriculture
DOI Number:	https://doi.org/10.1007/s10327-011-0322-1
Publisher:	Springer
Keywords:	Vegetables; Lady’s fingers; Bacterial soft rot; Identification.
Depositing User:	Nur Farahin Ramli
Date Deposited:	22 Jan 2014 06:53
Last Modified:	10 Dec 2015 03:38
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10327-011-0322-1
URI:	http://psasir.upm.edu.my/id/eprint/23725
Statistic Details:	View Download Statistic

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