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Morphology, flow cytometry and molecular assessment of ex-vitro grown micropropagated anthurium in comparison with seed germinated plants


Citation

Gantait, Saikat and Sinniah, Uma Rani (2011) Morphology, flow cytometry and molecular assessment of ex-vitro grown micropropagated anthurium in comparison with seed germinated plants. African Journal of Biotechnology, 10 (64). art. no. 56A934C35652. pp. 13991-13998. ISSN 1684–5315

Abstract

Micropropagated and seed propagated plantlets of anthurium (Anthurium andreanum Lind. cv. CanCan) were transferred to similar field condition and growth stage. A comparative study was conducted using morphological parameters, ploidy level assessment as well as deoxyribonucleic acid (DNA) fingerprinting using inter simple sequence repeat (ISSR) markers. The in vitro generated anthurium plants exhibited comparable vegetative growth and more sucker production when compared to plants propagated through seeds. No variation in ploidy level wasestablished through flow cytometric study. Genetic assessment through ISSR showed no polymorphism in banding pattern. It was revealed that there was no significant variation between micropropagated and seed propagated plants at ploidy and molecular level assuring the trueness of the micropropagated anthurium clones and their commercial applicability.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number: https://doi.org/10.5897/AJB11.1855
Publisher: Academic Journals
Keywords: Anthurium andreanum Lind; Ex vitro performance; ISSR; Morphological competence; Ploidy level
Depositing User: Nur Farahin Ramli
Date Deposited: 28 Nov 2013 17:22
Last Modified: 31 May 2016 07:33
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJB11.1855
URI: http://psasir.upm.edu.my/id/eprint/23680
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