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Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.


Citation

Muhammad Noor, Ahmad Shukri and Inami, W. and Miyakawa, Atsuo and Kawata, Yoshimasa (2011) Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. International Journal of the Physical Sciences, 6 (33). art. no. D06240B19089 . 7435 -7440. ISSN 1992 -1950

Abstract

Three emission wavelengths from two-photon excitation techniques were use to determinefree Ca2+ using a new analytical calculation. This was achieved by considering theinteraction of protein with free Ca2+ and indo-1 in protein contaminated sample. The emissions obtained from the excitation with the dissociation constants were used in the calculation. Agreement values of the Ca2+ from the calculation with the known Ca2+ buffer solution used for the measurement was obtained for various Ca2+ and protein concentration. The analytical analysis shows that dissociation constants for each binding’sdeviation errors are small to affect free Ca2+ determination. This would provide an accurate measurement of free Ca2+ in biological cells than the typical two emission wavelength processes. Furthermore, using two-photon excitation methods, deep inside cells observation of free Ca2+ are possible using the methods introduced.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
DOI Number: https://doi.org/10.5897/IJPS11.482
Keywords: Free Ca2+; indo-1; two-photon excitation methods.
Depositing User: Muizzudin Kaspol
Date Deposited: 10 Sep 2014 01:24
Last Modified: 20 Oct 2015 05:59
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/IJPS11.482
URI: http://psasir.upm.edu.my/id/eprint/23367
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