Citation
Chew, Few Ne and Tan, Wen Siang and Tey, Beng Ti
(2011)
Fluorescent quantitation method for differentiating the nativity of green fluorescent protein.
Journal of Bioscience and Bioengineering, 111 (2).
pp. 246-248.
ISSN 1389-1723; ESSN: 1347-4421
Abstract
A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands which is unable to be detected using the spectrofluorometric method.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Engineering Institute of Bioscience |
| DOI Number: | https://doi.org/10.1016/j.jbiosc.2010.10.004 |
| Publisher: | The Society for Biotechnology, Japan |
| Keywords: | Degradation; Denaturation; Fluorescence quantitation; Gel imaging method; Green fluorescent protein; Nativity; Spectrofluorometer |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 07 Dec 2015 02:44 |
| Last Modified: | 07 Dec 2015 02:44 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jbiosc.2010.10.004 |
| URI: | http://psasir.upm.edu.my/id/eprint/23106 |
| Statistic Details: | View Download Statistic |
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