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Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis


Citation

Go, Wen Ze and H'ng, Paik San and Wong, Mui Yun and Chin, Kit Ling and Ujang, Salmiah and Noran, Aizat Shamin (2019) Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis. Archives of Phytopathology and Plant Protection, 52 (7-8). pp. 639-666. ISSN 0323-5408; ESSN: 1477-2906

Abstract

White root disease caused by Rigidoporus microporus is the most devastating disease in majority of the rubber growing countries, including Malaysia. This study aimed to screen and decipher the mechanisms involved in the biocontrol agents responsible for the inhibition of R. microporus. Among 16 fungal isolates, Trichoderma spp. showed promising results with the highest percent of inhibition shown by Trichoderma asperellum (80.54%). Scanning electron microscopy study revealed coiling of hyphae by Trichoderma species against R. microporus. T. asperellum has demonstrated a maximum inhibition in both volatile and non-volatile metabolite tests with its 75% culture filtrate on PDA plate was observed to cause abnormal morphological character in R. microporus. All Trichoderma species were shown to produce hydrolytic enzymes (chitinase, cellulase and β-1,3-glucanase) and they were active siderophore producers. Present study demonstrated the possible mechanisms involved and responsible for successful inhibition of R. microporus under in vitro condition especially by T. asperellum.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Forestry
Institute of Plantation Studies
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.1080/03235408.2019.1587821
Publisher: Informa UK
Keywords: White root rot; Mycoparasitism; Antibiosis; Enzymatic activity; Siderophores; Trichoderma spp.
Depositing User: Nabilah Mustapa
Date Deposited: 05 Mar 2020 06:33
Last Modified: 05 Mar 2020 06:33
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/03235408.2019.1587821
URI: http://psasir.upm.edu.my/id/eprint/22670
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