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Synthesis of medium-chain glycerides using lipase from Candida rugosa


Citation

Wong, W. C. and Basri, Mahiran and Abdul Razak, Che Nyonya and Salleh, Abu Bakar (2000) Synthesis of medium-chain glycerides using lipase from Candida rugosa. Journal of the American Oil Chemists' Society, 77 (1). pp. 85-88. ISSN 0003-021X; ESSN: 1558-9331

Abstract

Enzymatic synthesis of medium-chain glycerides (MCG) from capric acid and glycerol was studied using lipase from Candida rugosa. The effects of various reaction parameters such as time, molar ratio of substrates (mmol capric acid/mmol glycerol), amount of lipase, type of organic solvents, and initial water activity (a w ) were studied. The best conditions tested for MCG synthesis at 37°C were, respectively, time, 24 h; molar ratio of substrates, 2.5; and amount of lipase, 100.0 mg. The use of organic solvents greatly influenced the activity of lipase in the synthesis of MCG. Generally, activity of lipase was high in nonpolar solvents with log P values from 3.50 to 4.50, where P is the partition coefficient between water and 1-octanol. The enzymatic synthesis of MCG was preferably carried out at an initial a w of 0.328, which resulted in maximal yield. Analysis of the products of reaction using gas chromatography showed that lipase from Candida rugosa seemed to produce more dicaprin and tricaprin than monocaprin.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science and Environmental Studies
DOI Number: https://doi.org/10.1007/s11746-000-0013-9
Publisher: AOCS Press
Keywords: Esterification; Lipase; Medium-chain glycerides; Solvents; Water activity
Depositing User: Nabilah Mustapa
Date Deposited: 02 Feb 2016 04:04
Last Modified: 05 Aug 2024 07:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11746-000-0013-9
URI: http://psasir.upm.edu.my/id/eprint/22570
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