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Lactate dehydrogenase in the guppy fish (Poecilia reticulata) as a biomarker of heavy-metal pollution in freshwater ecosystems


Citation

Yap, Chee Kong and Chong, Chou Min and Tan, Soon Guan (2011) Lactate dehydrogenase in the guppy fish (Poecilia reticulata) as a biomarker of heavy-metal pollution in freshwater ecosystems. Journal of Sustainability of Science and Management, 6 (2). pp. 240-246. ISSN 1823-8556

Abstract

Heavy metal concentrations and allozyme variations were determined in females of guppy fish (Poecilia reticulata) populations collected from polluted and unpolluted sites. the concentrations of Cu and Fe were significantly (P<0.05) higher in guppies collected from polluted drainage compared to the unpolluted population. Higher concentrations of Cu, Fe and Zn (P<0.05)were found in the surface sediment, indicating contamination by the three metals in the polluted drainage. The insignificant difference (P>0.05) in the Zn concentrations between the polluted and the unpolluted populations indicated that Zn, as a major essential metal, was regulated in this freshwater fish. seven enzyme systems EST, G6PDH, LDH, MDH, PGI, PGM, and SDH were tested. Only LDH (lactate dehydrogenase) was found to be agood biomarker for the contamination of Cu and Fe in P. reticulata. The zymogram of teh unpolluted wild population showed the same monomorphic allele as the unpolluted domesticated guppies from a pet shop,thus, further confirming LDH in P. reticulata as agood biomarker of contamination by Cu.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Publisher: Universiti Malaysia Terengganu
Keywords: Poecilia reticulata; Allozyme; Heavy metals; Biomarker
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 01 Sep 2015 02:21
Last Modified: 27 Oct 2015 03:34
URI: http://psasir.upm.edu.my/id/eprint/22329
Statistic Details: View Download Statistic

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