Citation
Zee, Hong Goh and Tan, Soon Guan and Bhassu, Subha and Tan, Wen Siang
(2011)
Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria.
Journal of Virological Methods, 175 (1).
pp. 74-79.
ISSN 0166-0934
Abstract
Macrobrachium rosenbergii nodavirus (MrNv) infects giant freshwater prawns and causes white tail disease (WTD). The coding region of the capsid protein of MrNv was amplified with RT-PCR and cloned into the pTrcHis2-TOPO vector. The recombinant plasmid was introduced into Escherichia coli and protein expression was induced with IPTG. SDS-PAGE showed that the recombinant protein containing the His-tag and myc epitope has a molecular mass of about 46kDa and it was detected by the anti-His antibody in Western blotting. The protein was purified using immobilized metal affinity chromatography (IMAC) and transmission electron microscopic analysis revealed that the recombinant protein assembled into VLPs.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Science |
| DOI Number: | https://doi.org/10.1016/j.jviromet.2011.04.021 |
| Publisher: | Elsevier |
| Keywords: | Virus-like particles; Nodavirus; Nodaviridae; Cloning; Purification; Expression |
| Depositing User: | Najwani Amir Sariffudin |
| Date Deposited: | 10 Feb 2015 01:11 |
| Last Modified: | 23 Nov 2015 02:58 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jviromet.2011.04.021 |
| URI: | http://psasir.upm.edu.my/id/eprint/22325 |
| Statistic Details: | View Download Statistic |
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