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Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids


Citation

Esa, Yuzine and Siraj, Siti Shapor and Abdul Rahim, Khairul Adha and Daud, Siti Khalijah and Ho, Ghim Chong and Tan, Soon Guan and Ismail, Mohammad Fadhil Syukri (2011) Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids. Sains Malaysiana, 40 (10). pp. 1087-1095. ISSN 0026-6039

Abstract

This study examined the genetic characteristics of twenty-six microsatellite primers developed from three cyprinid fishes(Cyprinus carpio Linnaeus,Barbus barbus Linnaeus and Barbonymus gonionotus Bleeker) in two indigenous mahseer. The Tor douronensis Valenciennes were randomly collected from two locations in Sarawak (N=52), while Tor tambroides Bleeker were obtained from Peninsular Malaysia (N=56). A total of ten and twelve primers were successfully amplified producing four and five polymorphic loci in T. douronensis and T. tambroides, respectively. The number of alleles perlocus ranging from 2 to 5 in T.douronensis and 2 to 7 in T. tambroides. A significant deviation from Hardy-Weinberg equilibrium (HWE) was observed at three loci (Barb37, Barb59 and Barb62) in one or more populations in T. tambroides while two loci (Barb37 and Barb62) were deviated in T. douronensis population of Batang Ai. Population structure analysis showed low level of inter-population genetic differentiation in both mahseer. Overall, the identified microsatellite loci should be useful in analysing T. douronensis and T. tambroides natural populations.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Agriculture
Faculty of Science
Publisher: Penerbit Universiti Kebangsaan Malaysia
Keywords: Cross-species study; Genetic characterization; Mahseer; Microsatellites
Depositing User: Najwani Amir Sariffudin
Date Deposited: 25 Aug 2014 02:15
Last Modified: 17 Oct 2018 04:31
URI: http://psasir.upm.edu.my/id/eprint/22302
Statistic Details: View Download Statistic

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