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Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42


Citation

Khusaini, Mohd Saif and Raja Abdul Rahman, Raja Noor Zaliha and Mohamad Ali, Mohd Shukuri and Leow, Adam Thean Chor and Basri, Mahiran and Salleh, Abu Bakar (2011) Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67 (3). pp. 401-403. ISSN 1744-3091

Abstract

An organic solvent-tolerant lipase from Bacillus sp. strain 42 was crystallized using the capillary-tube method. The purpose of studying this enzyme was in order to better understand its folding and to characterize its properties in organic solvents. By initially solving its structure in the native state, further studies on protein-solvent interactions could be performed. X-ray data were collected at 2.0 Å resolution using an in-house diffractometer. The estimated crystal dimensions were 0.09 0.19 0.08 mm. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 117.41, b = 80.85, c = 99.44 Å, β = 96.40°.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Bioscience
DOI Number: https://doi.org/10.1107/S1744309111002028
Publisher: International Union of Crystallography
Keywords: Counter diffusion; 42 lipase; Bacillus sp. strain 42; lipases; Organic solvent tolerance
Depositing User: Nur Farahin Ramli
Date Deposited: 31 Dec 2013 03:50
Last Modified: 28 Sep 2016 01:48
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1107/S1744309111002028
URI: http://psasir.upm.edu.my/id/eprint/22266
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