Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.

The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2 + (49%). The Mn-deficient enzyme was more sensitive to manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme. However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient enzyme achieved a maximum activity of 105% of control at 2.0mM.

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