The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis

Citation

O. S., Selvaraj and M., Sekaran and J. M., Pandaran and T. K., Mukherjee and Tan, S. G. and Barker, J. S. F. (1990) The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis. In: 15th Malaysian Biochemical Society Conference, 3 - 4 Sept. 1990, Universiti Kebangsaan Malaysia, Bangi, Selangor. (pp. 166-169). (Unpublished)

Abstract

The presence of KODAK Photoflo-600 (Cat.No.4055786),which contains ethylene glycol,in the cellulose acetate electrophoresis (CAE) buffers enhances the activities and sharpens the bands formed by several goat and buffalo blood enzymes on gels. This solution also overcame the problems encountered with overheating in some buffer systems. Its presence in the gel buffer also made it easier to soak the gel strips without any entrapment of air bubles. Malic enzyme (ME),malate dehydrogenase (MDH). 6-Phosphoglucose dehydrogenase(6pgdh). Glucose phosphate isomerase (GPI). Isocitrate dehydrogenase (IDH) and NADH-Diaphorase (NADH-DIA) from both buffaloes and goats exhibited ,arked improvement in band resolution and staining properties.

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Additional Metadata

Item Type:	Conference or Workshop Item (Paper)
Divisions:	Faculty of Science
Keywords:	Electrophoresis; Ethylene glycol;Buffer solutions
Depositing User:	Erni Suraya Abdul Aziz
Date Deposited:	27 Feb 2015 03:06
Last Modified:	14 May 2015 08:32
URI:	http://psasir.upm.edu.my/id/eprint/19363
Statistic Details:	View Download Statistic

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