Citation
Tan, Geok Hun and Yusoff, Khatijah and Seow, Heng Fong and Tan, Wen Siang
(2005)
Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7.
Journal of Medical Virology, 77 (4).
pp. 475-480.
ISSN 0146-6615
Abstract
The immunodominant region of hepatitis B virus (HBV) located in the viral small surface antigen (S-HBsAg) elicits virus-neutralizing and protective antibodies. In order to develop an easy and inexpensive method to produce this region without the need for extensive purification, amino acid residues 111-156 of S-HBsAg were fused to the C-terminal end of the 10B capsid protein of T7 phage. Western blotting and ELISA confirmed the expression of the recombinant protein on the surface of the phage particles. The recombinant phage exhibited the antigenic and immunogenic characteristics of HBsAg, illustrating its potential as an immunological reagent and vaccine. © 2005 Wiley-Liss, Inc.
Download File
Official URL or Download Paper: http://dx.doi.org/10.1002/jmv.20479
|
Additional Metadata
| Item Type: | Article |
|---|---|
| Subject: | Hepatitis B virus |
| Subject: | Immunology |
| Subject: | Recombinant proteins |
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences |
| DOI Number: | https://doi.org/10.1002/jmv.20479 |
| Keywords: | HBsAg; HBV; Immunodominant region; Immunology; Phage display; T7 phage |
| Depositing User: | Azwana Abdul Rahman |
| Date Deposited: | 26 Oct 2011 14:27 |
| Last Modified: | 21 Jan 2016 02:00 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jmv.20479 |
| URI: | http://psasir.upm.edu.my/id/eprint/18325 |
| Statistic Details: | View Download Statistic |
Actions (login required)
 |
View Item |