The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli

Citation

Saadun, Rafidah and Wen, Siang Tan and Omar, Abdul Rahman and Bejo, Mohd. Hair and Eshaghi, Majid and Yusoff, Khatijah (2008) The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli. Pertanika Journal of Tropical Agricultural Science, 31 (1). pp. 91-99. ISSN 1511-3701

Abstract

The hypervariable region (HVR) of VP2 protein of infectious bursal disease virus (IBDV) elicits neutralising antibodies, but it is highly hydrophobic and tends to form inclusion bodies when expressed in Escherichia coli. To improve its solubility, the VP2(HVR) was fused to the C-terminal end of Newcastle disease virus (NDV) nucleocapsid (NP) protein and expressed in E. coli TOP 10 cells under the control of trc promoter. However, the fusion protein, NP-VP2(HVR)-trc, aggregated into insoluble inclusion bodies in the host cells. Therefore the coding region of NP-VP2(HVR) was sub-cloned into expression vectors containing the T7 promoter. The solubility of the NP-VP2(HVR)-T7 fusion proteins improved dramatically in E. coli BL21 (DE3), BL21 (SI) and Origami B cells.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Biotechnology and Biomolecular Sciences
Publisher:	Universiti Putra Malaysia Press
Keywords:	Newcastle disease virus; Infectious bursal disease virus; VP2 solubility; Hypervariable region.
Depositing User:	Ms. Nida Hidayati Ghazali
Date Deposited:	02 Jul 2013 02:20
Last Modified:	18 Sep 2015 03:01
URI:	http://psasir.upm.edu.my/id/eprint/17251
Statistic Details:	View Download Statistic

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