Citation
Choong, Yew Keong and Mohamed Mustapha, Noordin and Mohamed, Suhaila and Ali, Abdul Manaf and Umar, Nor Aini and Tong, Chow Chin
(2008)
The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts.
International Journal of Medicinal Mushrooms, 10 (2).
pp. 115-125.
ISSN 1521-9437; ESSN: 1940-4344
Abstract
The cytotoxic effect of crude extracts of three Ganoderma species (G. lucidum, G. tropicum, and G. tsugae) was screened against the human estrogen-independent cell line, MDA-MB-435 using a microculture tetrazolium (MTT) assay. The crude extract obtained from the fruiting body of Ganoderma lucidum by hot water extraction procedures had IC50 values ranging from 140 to 350 μg mL−1. Acridine-orange/propidium iodine staining, electron microscopy, and transmission electron microscopy showed that the crude extract caused both apoptosis and necrosis. The G. lucidum mycelial crude extract was most effective, with an IC50 value of 148 μg mL−1.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Food Science and Technology Faculty of Veterinary Medicine |
| DOI Number: | https://doi.org/10.1615/IntJMedMushr.v10.i2.20 |
| Publisher: | Begell House |
| Keywords: | Apoptosis; Ganoderma; IC50; Ling Zhi mushrooms; MDA-MB-435; Medicinal mushrooms; Mycelium; Necrosis |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 08 Apr 2016 07:34 |
| Last Modified: | 08 Apr 2016 07:34 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1615/IntJMedMushr.v10.i2.20 |
| URI: | http://psasir.upm.edu.my/id/eprint/17248 |
| Statistic Details: | View Download Statistic |
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