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Severity of asthma: the role of CD25+,CD30+, NF-κB, and apoptotic markers


Citation

Abdulamir, Ahmed Sahib and Kadhim, Haider Sabah and Hafidh, Rand Riadh and Ali, M. A. and Faik, I. and Abu Bakar, Fatimah and Abbas, Kassim Ali (2009) Severity of asthma: the role of CD25+,CD30+, NF-κB, and apoptotic markers. Journal of Investigational Allergology and Clinical Immunology, 19 (3). pp. 218-224. ISSN 1018-9068; ESSN: 1698-0808

Abstract

Objectives: We studied the role of the regulatory T cells CD4+CD25+ (Treg) and activated CD4+CD30+ cells in the pathogenesis of asthma and their association with apoptosis and NF-κB in patients with mild intermittent asthma (MA), severe persistent asthma (SA), and healthy volunteers (HV). Methods: Peripheral blood lymphocytes (PBL) were extracted from asthmatic patients during exacerbations, and CD4+ cells were separated using Dynal beads. Immunostaining of whole PBL for NF-κB, Bax, and Bcl-2, and immunostaining of CD4+ cells for CD25+ and CD30+ cells were performed using immunocytochemistry. Results: Treg cells were expressed at higher levels in MA than in HV and SA (P<.05), while CD30+ T cells were expressed at higher levels in both SA and MA than in HV (P<.05), although there was no remarkable difference between SA and MA (P>.05). Levels of NF-κB, Bcl-2, and Bcl-2/Bax increased, whereas those of Bax decreased, progressively, from MA to SA (P<.05). NF-κB levels correlated directly with the Bcl-2/Bax ratio and with CD4+CD30+ cells in SA and MA, whereas CD4+CD30+ cells correlated inversely with the Bcl-2/Bax ratio. Conclusions: Unregulated Treg cells probably return inflammatory responses to normal values during exacerbations in MA; however, expression of Treg cells was extensively diminished in SA, leading to probable loss of suppressive control over underlying immune reactions. CD4+CD30+ cells were associated with the pathogenesis of asthma but not with severity. NF-κB seems to be the central inflammatory factor in SA, with a remarkable loss of PBL apoptosis, diminished Treg levels, and high CD30+ cell levels that probably induce NF-κB, which in turn blocks the proapoptotic potential of CD30 induction itself.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Institute of Bioscience
Publisher: Esmon Publicidad
Keywords: Asthma; Apoptosis; Memory cells; CD45RO; TH1; TH2; IL-4; IFN-γ
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 08 Jan 2014 00:55
Last Modified: 29 Nov 2019 01:22
URI: http://psasir.upm.edu.my/id/eprint/16687
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