Citation
Abstract
Objectives: We studied the role of the regulatory T cells CD4+CD25+ (Treg) and activated CD4+CD30+ cells in the pathogenesis of asthma and their association with apoptosis and NF-κB in patients with mild intermittent asthma (MA), severe persistent asthma (SA), and healthy volunteers (HV). Methods: Peripheral blood lymphocytes (PBL) were extracted from asthmatic patients during exacerbations, and CD4+ cells were separated using Dynal beads. Immunostaining of whole PBL for NF-κB, Bax, and Bcl-2, and immunostaining of CD4+ cells for CD25+ and CD30+ cells were performed using immunocytochemistry. Results: Treg cells were expressed at higher levels in MA than in HV and SA (P<.05), while CD30+ T cells were expressed at higher levels in both SA and MA than in HV (P<.05), although there was no remarkable difference between SA and MA (P>.05). Levels of NF-κB, Bcl-2, and Bcl-2/Bax increased, whereas those of Bax decreased, progressively, from MA to SA (P<.05). NF-κB levels correlated directly with the Bcl-2/Bax ratio and with CD4+CD30+ cells in SA and MA, whereas CD4+CD30+ cells correlated inversely with the Bcl-2/Bax ratio. Conclusions: Unregulated Treg cells probably return inflammatory responses to normal values during exacerbations in MA; however, expression of Treg cells was extensively diminished in SA, leading to probable loss of suppressive control over underlying immune reactions. CD4+CD30+ cells were associated with the pathogenesis of asthma but not with severity. NF-κB seems to be the central inflammatory factor in SA, with a remarkable loss of PBL apoptosis, diminished Treg levels, and high CD30+ cell levels that probably induce NF-κB, which in turn blocks the proapoptotic potential of CD30 induction itself.
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Official URL or Download Paper: http://www.jiaci.org/summary/vol19-issue3-num470
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Food Science and Technology Institute of Bioscience |
Publisher: | Esmon Publicidad |
Keywords: | Asthma; Apoptosis; Memory cells; CD45RO; TH1; TH2; IL-4; IFN-γ |
Depositing User: | Khairil Ridzuan Khahirullah |
Date Deposited: | 08 Jan 2014 00:55 |
Last Modified: | 29 Nov 2019 01:22 |
URI: | http://psasir.upm.edu.my/id/eprint/16687 |
Statistic Details: | View Download Statistic |
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