Citation
Abstract
Background and Aim: Nigella sativa and its active constituent thymoquinone (TQ) have been exploited for their various health benefits. This work was aimed to investigate the regulatory effects of TQ-rich fraction (TQRF) and commercial TQ on the low-density lipoprotein receptor (LDLR) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) genes in HepG2 cells. Methods and Results: TQRF was extracted from N. sativa seeds using supercritical fluid extraction. The regulatory effects of TQRF at 80 μg/ml and TQ at 2 μg/ml on LDLR and HMGCR gene expression were investigated in HepG2 cells using quantitative real-time PCR. The TQ content in TQRF was 2.77% (w/w) and was obtained at a temperature of 40°C and a pressure of 600 bar. Treatment of cells with TQRF and TQ resulted in a 7- and 2-fold upregulation of LDLR mRNA level, respectively, compared with untreated cells. The mRNA level of HMGCR was downregulated by 71 and 12%, respectively, compared with untreated cells. Conclusion: TQRF and TQ regulated genes involved in cholesterol metabolism by two mechanisms, the uptake of low-density lipoprotein cholesterol via the upregulation of the LDLR gene and inhibition of cholesterol synthesis via the suppression of the HMGCR gene.
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Official URL or Download Paper: https://www.karger.com/Article/Abstract/227264
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Medicine and Health Science Faculty of Veterinary Medicine Institute of Bioscience |
DOI Number: | https://doi.org/10.1159/000227264 |
Publisher: | Karger |
Keywords: | 25-Hydroxycholesterol; 3-Hydroxy-3-methyl-glutaryl-coenzyme A reductase; HepG2 cells; Low-density lipoprotein receptor; Nigella sativa; Supercritical fluid extraction; Thymoquinone; Thymoquinone-rich fraction |
Depositing User: | Raja Norazlinda Raja Azenam |
Date Deposited: | 15 May 2013 06:25 |
Last Modified: | 30 Oct 2019 08:51 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1159/000227264 |
URI: | http://psasir.upm.edu.my/id/eprint/16479 |
Statistic Details: | View Download Statistic |
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