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Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR


Ponniah, Jeyaletchumi and Robin, Tunung and S. P., Margaret and Radu, Son and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Nishibuchi, Mitsuaki and Nakaguchi, Yoshitsugu and Malakar, Pradeep Kumar (2010) Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR. International Food Research Journal, 17 (2). pp. 281-286. ISSN 1985-4668; ESSN: 2231-7546


The aim of this study was to assess the most probable number-polymerase chain reaction (MPN-PCR) technique for detection of Listeria monocytogenes in salad vegetables in comparison with reference EN ISO 11290-2 and Food Drug Administration Bacteriological Analytical Manual method using artifcial and naturally contaminated samples. Based on recovery of L. monocytogenes from artifcially contaminated samples, MPN-PCR showed a moderate correlation (R=0.67) between spiking concentration and microbial levels which was better than the FDA-BAM method (R=0.642) and ISO 11290-2:1998 method (R=0.655). With naturally contaminated samples, it was found that L. monocytogenes was detected in 25% of the vegetable samples using MPN-PCR; 15% of the samples by the FDA-BAM method and 8% of samples using ISO 11290-2:1998 method. Overall, MPN-PCR was found to be a rapid and reliable method that could facilitate the enumeration of L. monocytogenes in vegetables.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Listeria monocytogenes; Enumeration; Salad vegetables; MPN-PCR
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 26 Dec 2012 08:35
Last Modified: 25 Sep 2015 07:54
URI: http://psasir.upm.edu.my/id/eprint/16411
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