Citation
Abstract
The aim of this study was to assess the most probable number-polymerase chain reaction (MPN-PCR) technique for detection of Listeria monocytogenes in salad vegetables in comparison with reference EN ISO 11290-2 and Food Drug Administration Bacteriological Analytical Manual method using artifcial and naturally contaminated samples. Based on recovery of L. monocytogenes from artifcially contaminated samples, MPN-PCR showed a moderate correlation (R=0.67) between spiking concentration and microbial levels which was better than the FDA-BAM method (R=0.642) and ISO 11290-2:1998 method (R=0.655). With naturally contaminated samples, it was found that L. monocytogenes was detected in 25% of the vegetable samples using MPN-PCR; 15% of the samples by the FDA-BAM method and 8% of samples using ISO 11290-2:1998 method. Overall, MPN-PCR was found to be a rapid and reliable method that could facilitate the enumeration of L. monocytogenes in vegetables.
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Official URL or Download Paper: http://www.ifrj.upm.edu.my/17%20%2802%29%202010/IF...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Food Science and Technology Faculty of Medicine and Health Science |
| Publisher: | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| Keywords: | Listeria monocytogenes; Enumeration; Salad vegetables; MPN-PCR |
| Depositing User: | Khairil Ridzuan Khahirullah |
| Date Deposited: | 26 Dec 2012 08:35 |
| Last Modified: | 25 Sep 2015 07:54 |
| URI: | http://psasir.upm.edu.my/id/eprint/16411 |
| Statistic Details: | View Download Statistic |
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