Citation
Abstract
Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium phosphate and sodium citrate), were evaluated for their effectiveness in lipase recovery. The stability of lipase in each of the solutions was tested, and phase diagrams were constructed for each system. The optimum partition efficiency for the purification of lipase was obtained in an ATPS of 16% (w/w) 2-propanol and 16% (w/w) phosphate in the presence of 4.5% (w/v) NaCl. The purified lipase had a purification factor of 13.5 and a yield of 99%.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Engineering |
| DOI Number: | https://doi.org/10.1016/j.procbio.2009.05.008 |
| Publisher: | Elsevier |
| Keywords: | Lipase; Aqueous two-phase system; Alcohol/salt system; Purification; Burkholderia pseudomallei |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 25 Sep 2015 02:25 |
| Last Modified: | 25 Sep 2015 02:25 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.procbio.2009.05.008 |
| URI: | http://psasir.upm.edu.my/id/eprint/16396 |
| Statistic Details: | View Download Statistic |
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