Primary capture of high molecular weight nucleic acids using aqueous two-phase systems

Citation

Luechau, Frank and Ling, Tau Chuan and Lyddiatt, Andrew (2009) Primary capture of high molecular weight nucleic acids using aqueous two-phase systems. Separation and Purification Technology, 66 (1). pp. 202-207. ISSN 1383-5866; ESSN: 1873-3794

Abstract

The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (E. coli) cell paste. The process yielded a relatively protein-free pDNA solution with 89.5% pDNA recovery while low molecular weight RNA was partially removed. The results gained here led the way to an alternative process options for the processing of pDNA. A reproducible process involving two extraction steps to recover pDNA from partially disrupted E. coli cell paste was developed and is presented here.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Engineering
DOI Number:	https://doi.org/10.1016/j.seppur.2008.12.008
Publisher:	Elsevier
Keywords:	Selective partition; Plasmid DNA; ATPS; NaCl; E. coli cell paste
Depositing User:	Nurul Ainie Mokhtar
Date Deposited:	02 Aug 2015 07:47
Last Modified:	05 Oct 2015 05:53
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.seppur.2008.12.008
URI:	http://psasir.upm.edu.my/id/eprint/16338
Statistic Details:	View Download Statistic

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