UPM Institutional Repository

Optimized lipase catalyzed synthesis of adipate ester in a solvent-free system


Chaibakhsh, Naz and Abdul Rahman, Mohd Basyaruddin and Abd. Aziz, Suraini and Basri, Mahiran and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2009) Optimized lipase catalyzed synthesis of adipate ester in a solvent-free system. Journal of Industrial Microbiology & Biotechnology, 36 (9). pp. 1149-1155. ISSN 1367-5435; ESSN: 1476-5535


Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to important reaction parameters including time, temperature, agitation speed, and amount of enzyme. A high conversion yield was achieved using low enzyme amounts of 2.5% w/w at 60C, reaction time of 438 min, and agitation speed of 500 rpm. The good correlation between predicted value (96.0%) and actual value (95.5%) implies that the model derived from RSM allows better understanding of the effect of important reaction parameters on the lipase-catalyzed synthesis of adipate ester in an organic solvent-free system. Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester.

Download File

PDF (Abstract)
Optimized lipase catalyzed synthesis of adipate ester in a solvent.pdf

Download (83kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Bioscience
DOI Number: https://doi.org/10.1007/s10295-009-0596-x
Publisher: Springer
Keywords: Lipase; Adipate ester; Solvent-free system; Optimization; Response surface methodology
Depositing User: Nida Hidayati Ghazali
Date Deposited: 16 Jul 2012 04:52
Last Modified: 02 Sep 2016 04:39
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10295-009-0596-x
URI: http://psasir.upm.edu.my/id/eprint/15995
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item