Citation
Abstract
Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to important reaction parameters including time, temperature, agitation speed, and amount of enzyme. A high conversion yield was achieved using low enzyme amounts of 2.5% w/w at 60C, reaction time of 438 min, and agitation speed of 500 rpm. The good correlation between predicted value (96.0%) and actual value (95.5%) implies that the model derived from RSM allows better understanding of the effect of important reaction parameters on the lipase-catalyzed synthesis of adipate ester in an organic solvent-free system. Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Science Institute of Bioscience |
| DOI Number: | https://doi.org/10.1007/s10295-009-0596-x |
| Publisher: | Springer |
| Keywords: | Lipase; Adipate ester; Solvent-free system; Optimization; Response surface methodology |
| Depositing User: | Ms. Nida Hidayati Ghazali |
| Date Deposited: | 16 Jul 2012 04:52 |
| Last Modified: | 02 Sep 2016 04:39 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10295-009-0596-x |
| URI: | http://psasir.upm.edu.my/id/eprint/15995 |
| Statistic Details: | View Download Statistic |
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