Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM)

Citation

Husin, Nurdiyana and Mustapa Kamal, Siti Mazlina and M., Siti Nor Fadhilah (2008) Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM). International Journal of Engineering and Technology, 5 (1). pp. 48-56.

Abstract

The present study embarked on the objective of optimizing protein extraction from freeze dried fish waste (FD-FW), sardine (Sardina pilchardus). Introduction of freeze drying prior to extraction was aimed at reducing the risk of protein loses in fish waste (FW) during frozen storage before the extraction process. Response surface methodology (RSM) was used to study the effect of independent variables, namely time (30-60 minutes), pH (7-11), rotation speed (100-300 rpm), and NaOH: substrate ratio (1-3) on protein extraction from FD-FW. From RSM-generated model, the optimum conditions for extraction of protein from FD-FW were identified to be at pH 10.56 in 48.61 minutes reaction time, with rotation speed of 104.77 rpm and NaOH: substrate ratio of 1.54. Predicted protein yield was 85.02 mg/ml while an experimental protein yield was 83.51 mg/ml as revealed by confirmatory studies.

Download File

Preview

PDF (Abstract)
Optimization of protein extraction from freeze dried fish waste using response surface methodology.pdf
Download (83kB) | Preview

Additional Metadata

Item Type:	Article
Divisions:	Faculty of Engineering
Keywords:	Sardine pilchardus; Freeze drying; Freeze dried fish waste; Response surface methodology
Depositing User:	Fatimah Zahrah @ Aishah Amran
Date Deposited:	08 Dec 2014 04:59
Last Modified:	17 Sep 2015 01:09
URI:	http://psasir.upm.edu.my/id/eprint/15983
Statistic Details:	View Download Statistic

Actions (login required)

View Item