Citation
Yap, Wei Boon and Tey, Beng Ti and Ng, Michelle Yeen Tan and Ong, Swee Tin and Tan, Wen Siang
(2009)
N-terminally His-tagged hepatitis B core antigens: construction, expression, purification and antigenicity.
Journal of Virological Methods, 160 (1-2).
pp. 125-131.
ISSN 0166-0934; ESSN: 1879-0984
Abstract
The core antigen of the hepatitis B virus (HBcAg) has been used widely as a diagnostic reagent for the identification of the viral infection. However, purification using the conventional sucrose density gradient ultracentrifugation is time consuming and costly. To overcome this, HBcAg particles displaying His-tag on their surface were constructed and produced in Escherichia coli. The recombinant His-tagged HBcAgs were purified using immobilized metal affinity chromatography. Transmission electron microscopy and enzyme-linked immunosorbent assay (ELISA) revealed that the displayed His-tag did not impair the formation of the core particles and the antigenicity of HBcAg.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Engineering Institute of Bioscience |
| DOI Number: | https://doi.org/10.1016/j.jviromet.2009.04.038 |
| Publisher: | Elsevier |
| Keywords: | Hepatitis B capsid; Protein display; His-tag; Purification; Antigenicity |
| Depositing User: | Fatimah Zahrah @ Aishah Amran |
| Date Deposited: | 11 Nov 2014 01:55 |
| Last Modified: | 30 Nov 2016 08:09 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jviromet.2009.04.038 |
| URI: | http://psasir.upm.edu.my/id/eprint/15854 |
| Statistic Details: | View Download Statistic |
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