Citation
Ling, Tau Chuan and Lyddiatt, Andrew
(2006)
Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent.
Biotechnology and Bioprocess Engineering, 11 (1).
pp. 84-87.
ISSN 1226-8372; ESSN: 1976-3816
Abstract
Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency, at 6.5% and a specific activity of 10.2 IU/mg.
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Official URL or Download Paper: https://link.springer.com/article/10.1007/BF029318...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Engineering |
| DOI Number: | https://doi.org/10.1007/BF02931874 |
| Publisher: | The Korean Society for Biotechnology and Bioengineering |
| Keywords: | Dye-ligand; Cibacron Blue 3GA; G3PDH; Elution yield; Low ionic strength elution |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 04 Oct 2019 08:25 |
| Last Modified: | 04 Oct 2019 08:25 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/BF02931874 |
| URI: | http://psasir.upm.edu.my/id/eprint/15491 |
| Statistic Details: | View Download Statistic |
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