Isolation of trinucleotide microsatellite markers for Mystus nemurus

Citation

Hoh , Boon Peng and Siraj, Siti Shapor and Tan, Soon Guan and Yusoff, Khatijah (2008) Isolation of trinucleotide microsatellite markers for Mystus nemurus. Russian Journal of Genetics, 44 (3). pp. 369-371. ISSN 1022-7954

Abstract

Twelve single-locus trinucleotide microsatellite markers were developed to characterize the Asian river catfish, Mystus nemurus, an important food fish in Southeast Asia. They were obtained by using a rapid method, namely, the 5′ anchored PCR enrichment protocol. The specific primers were designed to flank the repeat sequences and these were subsequently used to characterize 90 unrelated fish from Malaysia. The number of alleles per locus ranged from 2 (MnVj2-281) to 12 (MnBp8-4-43b) while the levels of heterozygosity ranged from 0.0444 (MnVj2-1-19) to 0.7458 (MnVj2-291).

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Additional Metadata

Item Type:	Article
Subject:	Catfishes - Research
Subject:	Microsatellites (Genetics)
Subject:	Genetic markers
Divisions:	Faculty of Biotechnology and Biomolecular Sciences
Publisher:	Springer
Keywords:	Trinucleotide microsatellite
Depositing User:	Ms. Nida Hidayati Ghazali
Date Deposited:	16 Aug 2012 01:19
Last Modified:	17 Sep 2015 01:02
URI:	http://psasir.upm.edu.my/id/eprint/15335
Statistic Details:	View Download Statistic

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