Citation
Abstract
Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains. Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis. The optimal values of important factors were determined by response surface methodology (RSM). The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study. The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 mg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium. Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57 g/L and PABA 10 mM.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Institute of Bioscience |
| Publisher: | Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia |
| Keywords: | Folate; Lactic acid bacteria; Lactobacillus plantarum I-UL4; Response surface methodology |
| Depositing User: | Ms. Nida Hidayati Ghazali |
| Date Deposited: | 16 Aug 2012 03:00 |
| Last Modified: | 14 Aug 2017 03:35 |
| URI: | http://psasir.upm.edu.my/id/eprint/15142 |
| Statistic Details: | View Download Statistic |
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