Citation
Tam, Yew Joon and Allaudin, Zeenathul Nazariah and Rezaei, Morvarid Akhavan and Mohd Lila, Mohd Azmi and Bahaman, Abdul Rani and Lo, Sewn Cen and Tan, Joo Shun and Hani, Homayoun
(2016)
Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris.
Separation Science and Technology, 51 (5).
pp. 816-829.
ISSN 0149-6395; ESSN: 1520-5754
Abstract
Univariate screening on factors affecting the purification performance of recombinant hepatitis B surface antigen (HBsAg) on ion exchange chromatography (IEC) and size exclusion chromatography (SEC) and the establishment of a two-step purification strategy were performed. Amongst four IEC adsorbents examined, the use of Q Sepharose XL IEC adsorbent under optimized conditions together with optimized SEC purification was able to efficiently purify HBsAg. An established purification strategy comprising the two techniques further demonstrated adaptability for scale-up operations with a final total purification factor (PF) of 94.82 ± 16.20, HBsAg purity of 95.48% and recovery yield of 78.07%.
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Official URL or Download Paper: https://www.tandfonline.com/doi/abs/10.1080/014963...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Veterinary Medicine Institute of Bioscience |
| DOI Number: | https://doi.org/10.1080/01496395.2015.1135949 |
| Publisher: | Taylor & Francis |
| Keywords: | Gel filtration; Hepatitis B surface antigen; Ion exchange chromatography; Pichia pastoris; Protein purification strategy |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 09 May 2019 01:23 |
| Last Modified: | 09 May 2019 01:23 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/01496395.2015.1135949 |
| URI: | http://psasir.upm.edu.my/id/eprint/15080 |
| Statistic Details: | View Download Statistic |
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