Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers

Knowl­edge on genetic dis­tances and rela­tion­ships among breed­ing mate­ri­als has a sig­nif­i­cant impact on crop improve­ment. Mol­e­c­u­lar mark­ers are being used increas­ingly to deter­mine the genetic dis­tance between indi­vid­u­als. In this study, microsatel­lite mark­ers were used to esti­mate the genetic dis­tances between selected oil palm par­ent lines. Microsatel­lite mark­ers are highly reli­able, inher­ited in codom­i­nant fash­ion whereby het­erozy­gotes and homozy­gotes are dis­tin­guish­able, easy to score and can be rapidly pro­duced using PCR tech­nol­ogy. Nine microsatel­lite mark­ers were used to screen selected par­ent palms (15 duras and 4 pisiferas) and their prog­e­nies (16 DxP crosses). Data were scored and analysed using the Biosys-1 soft­ware to cal­cu­late the genetic dis­tance val­ues. A total of 29 poly­mor­phic bands were gen­er­ated. The genetic dis­tances between prog­e­nies ranged from 0.089 to 0.313. These results indi­cate that microsatel­lite mark­ers are pow­er­ful tools for study­ing genetic rela­tion­ships among DxP prog­e­nies. These mark­ers should be fur­ther explored to assist oil palm breeding.

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