Citation
Abdullah, Syahrilnizam and Wai Yeng, Wendy-Yeo and Hosseinkhani, Hossein and Hosseinkhani, Mohsen and Masrawa, Ehab and Ramasamy, Rajesh and Rosli, Rozita and Abdul Rahman, Sabariah and Domb, Abraham J.
(2010)
Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes.
Journal of Biomedicine and Biotechnology, 2010 (284840).
pp. 1-10.
ISSN 1110-7243, ESSN: 1110-7251
Abstract
A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines and in vivo through systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery. In vitro studies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12. In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery. Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression. The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range.
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