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Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation


Citation

Sekawi, Zamberi and Yusof, Rusmah and Shamsudin, Mariana Nor (2008) Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation. Research Journal of Microbiology, 3 (6). pp. 489-493. ISSN 1816-4935

Abstract

A study was conducted to portray a preliminary characteristic of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli in a local tertiary hospital in Malaysia. Sixteen clinical isolates of ESBLs producing E. coli from different sources were examined for blaSHV/TEM/CTX-M ESBL genes by PCR molecular assay. Each strain was found to carry at least one of the genes. This study demonstrated a high prevalence of blaCTX-M (81.3%) and blaTEM genes (75%). Only two strains (12.5%) carried the blaSHV gene. Nucleotide and deduced protein sequences determination showed; 61% produced CTX-M-15, 31% produced CTX-M-14 and 8% produced CTX-M-3. The antimicrobial susceptibility testing data determined that almost all sixteen isolates were resistant to oxyimino-cephalosporins, 46% resistant to gentamicin, 69% resistant to trimethoprim-sulfamethoxazole and 46% resistant to ciprofloxacin. This study preliminary emphasizes the epidemiology of the ESBL-producing E. coli particularly SHV, TEM and CTX-M-type producing E. coli in Malaysia.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3923/jm.2008.489.493
Publisher: Academic Journals
Keywords: TEM; SHV; CTX-M; ESBL; E. coli; Malaysia
Depositing User: Nabilah Mustapa
Date Deposited: 09 Apr 2019 03:46
Last Modified: 09 Apr 2019 03:46
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/jm.2008.489.493
URI: http://psasir.upm.edu.my/id/eprint/14675
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