Citation
Zain, Nurazah and Muse, Radzali and Ahmad, Syahida and Mahmood, Maziah
(2009)
Effects of plant growth regulators on callus induction from Cananga odorata flower petal explant.
African Journal of Biotechnology, 8 (12).
art. no. 00AA34E8081.
pp. 2740-2743.
ISSN 1684–5315
Abstract
The Cananga odorata callus was initiated from petals of the C. odorata flowers on MS medium and B5 vitamins containing 30 g/L sugar and 3 g/L agar. The medium was also supplemented with different concentrations of 1-naphtalene acetic acid (NAA), and combinations of NAA with 6-benzylaminpurine(BAP) as the plant growth regulators. It was observed that the C. odorata callus could be induced on media containing the combination of 3 mg/L NAA and 0.5 mg/L BAP. High concentration of NAA gave rise to pale, whitish and friable callus after 1 - 2 weeks of culture. The optimum pH for the cell culture was about 5.7 and incubation at 25 ± 2°C, in totally dark condition.
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Biotechnology and Biomolecular Sciences |
Publisher: | Academic Journals |
Keywords: | Cananga odorata; 1-naphtalene acetic acid; 6-benzylaminopurine; Callus culture |
Depositing User: | Ms. Nida Hidayati Ghazali |
Date Deposited: | 16 Aug 2012 07:59 |
Last Modified: | 14 Nov 2017 07:54 |
URI: | http://psasir.upm.edu.my/id/eprint/14401 |
Statistic Details: | View Download Statistic |
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