Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles

Citation

Abdul Manan, Norlida and Sieo, Chin Chin and Abdullah, Norhani and Ho, Yin Wan (2009) Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles. African Journal of Biotechnology, 8 (16). art. no. ED0B1398389. pp. 3964-3969. ISSN 1684–5315

Abstract

In the present study, distinctive RAPD fingerprints were generated for 12 Lactobacillus-probiotic strains from 5 Lactobacillus species (L. brevis, L. reuteri, L. gallinarium, L. salivarius and L. panis) after optimization of the RAPD parameters such as MgCl2, Taq polymerase, primer concentration and type of primer. The strains were differentiated under the same PCR protocol but different concentration of primer OPM-05 (50 pmole to differentiate the 5 L. brevis strains and 75 pmole to differentiate 2 strains of L. gallinarium, 3 strains of L. reuteri, a strain of L. panis and L. salivarius). The RAPD fingerprints generated could be differentiated by visual comparison of the profiles, without being analysed by relevant software. This allows specific, rapid, immediate and convenient identification of the Lactobacillus strains.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Biotechnology and Biomolecular Sciences Institute of Bioscience
Publisher:	Academic Journals
Keywords:	RAPD analysis; Lactobacillus; Differentiation
Depositing User:	Ms. Nida Hidayati Ghazali
Date Deposited:	12 Feb 2014 03:59
Last Modified:	09 Nov 2017 05:26
URI:	http://psasir.upm.edu.my/id/eprint/14071
Statistic Details:	View Download Statistic

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