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Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid


Citation

Hooi, Wei Yeng and Shamsudin, Mariana Nor and Abdul Rahim, Raha (2009) Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid. African Journal of Biotechnology, 8 (21). art. no. 84F18C88334. pp. 5621-5626. ISSN 1684–5315

Abstract / Synopsis

To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to be stably maintained in L. lactis MG1363 after transformation for at least 100 generations under non-selective conditions. The vector was also demonstrated to be able to express the gene coding for chloramphenicol acetyltransferase (cat) in L. lactis.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Institute of Bioscience
Publisher: Academic Journals
Keywords: Expression; Plasmid vector; Transformation
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 10 Sep 2014 09:04
Last Modified: 13 Nov 2017 10:37
URI: http://psasir.upm.edu.my/id/eprint/13757
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