Citation
Hooi, Wei Yeng and Shamsudin, Mariana Nor and Abdul Rahim, Raha
(2009)
Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid.
African Journal of Biotechnology, 8 (21).
art. no. 84F18C88334.
pp. 5621-5626.
ISSN 1684–5315
Abstract
To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to be stably maintained in L. lactis MG1363 after transformation for at least 100 generations under non-selective conditions. The vector was also demonstrated to be able to express the gene coding for chloramphenicol acetyltransferase (cat) in L. lactis.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Medicine and Health Science Institute of Bioscience |
| Publisher: | Academic Journals |
| Keywords: | Expression; Plasmid vector; Transformation |
| Depositing User: | Raja Norazlinda Raja Azenam |
| Date Deposited: | 10 Sep 2014 01:04 |
| Last Modified: | 13 Nov 2017 02:37 |
| URI: | http://psasir.upm.edu.my/id/eprint/13757 |
| Statistic Details: | View Download Statistic |
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