Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.

Citation

M.A., Bahmaninejad and Bejo, Mohd Hair and Omar, Abdul Rahman and Ideris, Aini and R., Toroghi (2008) Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran. Acta Virologica, 52 (3). pp. 167-174. ISSN 0001-723X

Abstract

Three infectious bursal disease viruses (IBDVs) were isolated from field outbreaks in IBDV-vaccinated and non-vaccinated layer chicken flocks. Agar gel precipitation test (AGPT), immunoperoxidase staining, transmission electron microscopy (TEM), inoculation into embryonated eggs, and chicken embryo fibroblasts (CEFs) confirmed that the isolates were IBDVs. RT-PCR, restriction fragment length polymorphism (RFLP), and phylogenetic analysis demonstrated that the isolates were very virulent IBDV (vvIBDV) and showed a nucleotide sequence similarity of 96.3 to 99.8% in comparison with other vvIBDV strains. It was concluded that the Iranian isolates represented vvIBDV of serotype 1 originating from Europe, Japan, and China.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Veterinary Medicine
Publisher:	Academic Electronic Press Ltd.
Keywords:	Infectious bursal disease virus; Restriction fragment length polymorphism; Sequence and phylogenetic analysis.
Depositing User:	Azizan Arshad
Date Deposited:	09 Dec 2013 04:38
Last Modified:	23 Oct 2015 07:35
URI:	http://psasir.upm.edu.my/id/eprint/13553
Statistic Details:	View Download Statistic

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