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Characterization of vancomycin-resistant Enterococcus isolates from broilers in Selangor, Malaysia.


Citation

Ghetachew, Yitbarek M. and Hassan, Latiffah and Zakaria, Zunita and Abdul Aziz, Saleha and Mat Isa, Kamaruddin and M.Z, Che Zalina (2009) Characterization of vancomycin-resistant Enterococcus isolates from broilers in Selangor, Malaysia. Tropical Biomedicine, 26 (3). pp. 280-288. ISSN 0127-5720

Abstract / Synopsis

Vancomycin-resistant Enterococcus (VRE) is an emerging nosocomial pathogen in humans. The use of antibiotics in human therapy and in the production of food animals has been incriminated in the emergence of this organism. The present study describes the distribution of VRE species, the vancomycin-resistant genes detected, the vancomycin resistance pattern observed, and the genetic diversity of the isolates found in live broiler chickens in Malaysia. Overall 140 VRE were isolated with species comprising Enterococcus faecalis (48%), Enterococcus faecium (25.7%), Enterococcus gallinarum (12.1%), Enterococcus casseliflavus (1.4%) and other Enterococcus species (12.8%). Vancomycin resistance gene vanA and intrinsic genes vanC1 and vanC2/3 were detected in the study population. VanA was detected in 15 (63.9%) of E. faecium, 23 (22.4%) of E. faecalis and in 3 (17.6%) E. gallinarum isolates. E-test was conducted on randomly selected 41 of the isolates and the minimum inhibition concentration (MIC) of vancomycin for five (11.9%) of tested isolates is more than 256µg/ml. Genotypic analysis using random amplified polymorphic DNA (RAPD) showed genetic diversity within the Enterococcus species.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Malaysian Society of Parasitology and Tropical Medicine
Keywords: Enterococcus; Vancomycin-resistant Enterococcus; Broiler; Selangor.
Depositing User: Azizan Arshad
Date Deposited: 09 Dec 2013 12:43
Last Modified: 27 Nov 2015 16:42
URI: http://psasir.upm.edu.my/id/eprint/13525
Statistic Details: View Download Statistic

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