Citation
Ghetachew, Yitbarek M. and Hassan, Latiffah and Zakaria, Zunita and Abdul Aziz, Saleha and Mat Isa, Kamaruddin and M.Z, Che Zalina
(2009)
Characterization of vancomycin-resistant Enterococcus isolates from broilers in Selangor, Malaysia.
Tropical Biomedicine, 26 (3).
pp. 280-288.
ISSN 0127-5720
Abstract
Vancomycin-resistant Enterococcus (VRE) is an emerging nosocomial pathogen in humans. The use of antibiotics in human therapy and in the production of food animals has been
incriminated in the emergence of this organism. The present study describes the distribution of VRE species, the vancomycin-resistant genes detected, the vancomycin resistance pattern observed, and the genetic diversity of the isolates found in live broiler chickens in Malaysia.
Overall 140 VRE were isolated with species comprising Enterococcus faecalis (48%), Enterococcus faecium (25.7%), Enterococcus gallinarum (12.1%), Enterococcus casseliflavus (1.4%) and other Enterococcus species (12.8%). Vancomycin resistance gene vanA and intrinsic genes vanC1 and
vanC2/3 were detected in the study population. VanA was detected in 15 (63.9%) of E. faecium, 23 (22.4%) of E. faecalis and in 3 (17.6%) E. gallinarum isolates. E-test was conducted on randomly selected 41 of the isolates and the minimum inhibition concentration (MIC) of vancomycin for
five (11.9%) of tested isolates is more than 256µg/ml. Genotypic analysis using random amplified polymorphic DNA (RAPD) showed genetic diversity within the Enterococcus species.
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