Citation
Tan, Cheau Yuaan and Raja Abdul Rahman, Raja Noor Zaliha and Abdul Kadir, Habsah and Tayya, Saad
(2010)
Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation.
African Journal of Biotechnology, 9 (46).
art. no. 9903FE419346.
pp. 7934-7941.
ISSN 1684–5315
Abstract
Guanidine hydrochloride (GdnHCl) denaturation of native and Ca-depleted Bacillus licheniformis α-amylase (BLA) was investigated both in the absence and presence of 2 mM calcium chloride (CaCl2)using circular dichroism, fluorescence spectroscopy and biological activity. In both states (Cadepleted and native form), the protein was denatured to a considerable extent in the absence of 2 mM
CaCl2 with concomitant loss of biological activity upon increasing GdnHCl concentration. On the other hand, this effect was significantly reduced when 2 mM CaCl2 was included in the incubation mixture as revealed by a higher relative mean residue ellipticity, higher relative fluorescence intensity, smaller change in emission maximum and lesser reduction in biological activity. Interestingly, using these probes, 2 mM CaCl2 seemed to offer the same degree of stability to Ca-depleted BLA as that observed
with native BLA in the absence of 2 mM CaCl2. All these results suggest calcium-induced stabilization of BLA against GdnHCl denaturation.
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