Citation
Khatib, Alfi and Hoe, Arie C. and Selamat, Jinap and Sarker, Zaidul Islam and Jaswir, Irwandi and Verpoorte, Robert
(2010)
Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity.
Journal of Liquid Chromatography and Related Technologies, 33 (2).
pp. 214-224.
ISSN 1082-6076; ESSN: 1520-572X
Abstract
We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase.
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