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Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic activity.


Citation

Khatib, Alfi and Hoek, Arie C. and Selamat, Jinap and Sarker, Zaidul Islam and Jaswir, Irwandi and Verpoorte, Robert (2010) Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic activity. Journal of Liquid Chromatography and Related Technologies, 33 (2). pp. 214-224. ISSN 1082-6076

Abstract

We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1080/10826070903439416
Publisher: Taylor & Francis
Keywords: Asthma; Centrifugal partition chromatography; Fingerprinting; Thin layer chromatography; Tracheospasmolytic assay; Vitex trifolia Linn.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 07 Jan 2014 05:15
Last Modified: 22 Sep 2015 06:55
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/10826070903439416
URI: http://psasir.upm.edu.my/id/eprint/13245
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