An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads

Citation

Lim, Crystale Siew Ying and Tung, Chee Hong and Rosli, Rozita and Chong, Pei Pei (2008) An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads. Journal of Microbiological Methods, 75 (3). pp. 576-578. ISSN 0167-7012; ESSN: 1872-8359

Abstract

This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Medicine and Health Science
DOI Number:	https://doi.org/10.1016/j.mimet.2008.07.026
Publisher:	Elsevier
Keywords:	Yeast; Cell wall disruption; Sorbitol lysis buffer; Glass beads; Total RNA isolation; Genomic DNA isolation; Candida
Depositing User:	Nurul Ainie Mokhtar
Date Deposited:	06 Jun 2015 11:27
Last Modified:	28 Mar 2017 14:04
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.mimet.2008.07.026
URI:	http://psasir.upm.edu.my/id/eprint/13008
Statistic Details:	View Download Statistic

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