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Aflatoxin in raw peanut kernels marketed in Malaysia.


Arzandeh, Sahar and Selamat, Jinap and Lioe, Hanifah Nuryani (2010) Aflatoxin in raw peanut kernels marketed in Malaysia. Journal of Food and Drug Analysis, 18 (1). pp. 44-50. ISSN 1021-9498


The occurrence of af latoxin in eighty-four samples of raw peanut kernels which are randomly collected from Malaysian super- markets was examined. Analysis for af latoxin was performed by solvent extraction and immunoaffinity clean-up followed by the determination using high performance liquid chromatography equipped with post-column photochemical reactor for enhanced detection and f luorescence detector. A detection limit of 0.01-0.09 ng/mL and a quantification limit of 0.04-0.30 ng/mL were obtained. The af latoxin concentrations ranged from not detected to 97.28 ng/g in all samples investigated. About 78.57% of the samples were contaminated with af latoxin, of which 10.71% exceeded the maximum tolerable limit of 15 ng/g set by the Codex. Average recoveries of the af latoxin analysis were acceptable which were in the range of 74.85 ± 8.83% for AFG2 at the concentration of 0.15 ng/mL and 103.91 ± 6.45% for AFB2 at the concentration of 0.15 ng/mL. The average daily intake estimated for total af la- toxins was 10.69 ng/kg body weight. There was a significant difference (P < 0.05) in af latoxin content between brands and locations.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: National Laboratories of Foods and Drugs
Keywords: Aflatoxin; Peanut; Food safety; HPLC with fluorescence detection; Immunoaffinity clean-up.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 08 Jan 2014 02:20
Last Modified: 27 Oct 2015 06:40
URI: http://psasir.upm.edu.my/id/eprint/12957
Statistic Details: View Download Statistic

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