Citation
Arzandeh, Sahar and Selamat, Jinap and Lioe, Hanifah Nuryani
(2010)
Aflatoxin in raw peanut kernels marketed in Malaysia.
Journal of Food and Drug Analysis, 18 (1).
pp. 44-50.
ISSN 1021-9498
Abstract
The occurrence of af latoxin in eighty-four samples of raw peanut kernels which are randomly
collected from Malaysian super-
markets was examined. Analysis for af latoxin was performed by solvent extraction and
immunoaffinity clean-up followed by the determination using high performance liquid chromatography
equipped with post-column photochemical reactor for enhanced detection and f luorescence detector.
A detection limit of 0.01-0.09 ng/mL and a quantification limit of 0.04-0.30 ng/mL were obtained.
The af latoxin concentrations ranged from not detected to 97.28 ng/g in all samples investigated.
About 78.57% of the samples were contaminated with af latoxin, of which 10.71% exceeded the maximum
tolerable limit of 15 ng/g set by the Codex. Average recoveries of the af latoxin analysis were
acceptable which were in the range of 74.85 ± 8.83% for AFG2 at the concentration of 0.15 ng/mL and
103.91 ± 6.45% for AFB2 at the concentration of 0.15 ng/mL. The average daily intake estimated for
total af la- toxins was 10.69 ng/kg body weight. There was a significant difference (P < 0.05) in
af latoxin content between brands and locations.
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