Citation
Ghaznavi-Rad, Ehsanollah and Shamsudin, Mariana Nor and Sekawi, Zamberi and Belkum, Alex van and Neela, Vasantha Kumari
(2010)
A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus.
Journal of Medical Microbiology, 59 (10).
pp. 1135-1139.
ISSN 0022-2615; ESSN: 1473-5644
Abstract
A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCCmec types that we tested were correctly characterized by our PCR assay. SCCmec type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type.
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Official URL or Download Paper: http://jmm.sgmjournals.org/content/59/10/1135.abst...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Medicine and Health Science |
| DOI Number: | https://doi.org/10.1099/jmm.0.021956-0. Epub 2010 Jul 8 |
| Publisher: | Society for General Microbiology |
| Keywords: | PCR assay; Staphylococcal cassette chromosome mec; Staphylococcus aureus |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 05 Jun 2015 11:18 |
| Last Modified: | 27 Nov 2015 03:00 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1099/jmm.0.021956-0. Epub 2010 Jul 8 |
| URI: | http://psasir.upm.edu.my/id/eprint/12859 |
| Statistic Details: | View Download Statistic |
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