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A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography


Citation

Ho, Chin Woi and Tan, Wen Siang and Chong, Fui Chin and Ling, Tau Chuan and Tey, Beng Ti (2009) A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography. Journal of Microbiology and Biotechnology, 19 (4). pp. 416-423. ISSN 1017-7825; ESSN: 1738-8872

Abstract

Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was first captured by anion exchange expanded bed adsorption chromatography integrated with a cell disruption process. This online capture process has shortened the process time and eliminated the “hold-up” period that may be detrimental to the quality of target protein. The eluted product from the expanded bed adsorption chromatography was subsequently purified using size-exclusion chromatography. The results showed that this novel purification protocol achieved a recovery yield of 45.1% with a product purity of 88.2%, which corresponds to a purification factor of 4.5. The recovered HBcAg is still biologically active as shown by ELISA test.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Institute of Bioscience
DOI Number: https://doi.org/10.4014/jmb.0804.254
Publisher: Korean Society for Microbiology and Biotechnology
Keywords: Hepatitis B core antigen; Escherichia coli; Online capture process; Expanded bed adsorption chromatography; Size-exclusion chromatography
Depositing User: Nabilah Mustapa
Date Deposited: 07 May 2015 02:52
Last Modified: 17 Sep 2015 00:10
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4014/jmb.0804.254
URI: http://psasir.upm.edu.my/id/eprint/12790
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